How is SY5Y different from SH?
Differentiation of SH-SY5Y cells relies on gradual serum deprivation; the addition of retinoic acid, neurotrophic factors and extracellular matrix proteins; and serial splitting to select for differentiated mature adherent neurons.
What is SH-SY5Y cell line?
SH-SY5Y is a human derived cell line used in scientific research. The original cell line, called SK-N-SH, from which it was subcloned was isolated from a bone marrow biopsy taken from a four-year-old female with neuroblastoma. SH-SY5Y cells are often used as in vitro models of neuronal function and differentiation.
What is neuroblastoma cell line?
Neuroblastoma (NB) cell lines are transformed, neural crest derived cells, capable of unlimited proliferation in vitro. These cell lines retain the ability of differentiation into neuronal cell types on treatment with various agents.
Are SH-SY5Y cells neurons?
SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line. It serves as a model for neurodegenerative disorders since the cells can be converted to various types of functional neurons by the addition of specific compounds.
What are SH-SY5Y neuroblastoma cells?
Description. SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line. It serves as a model for neurodegenerative disorders since the cells can be converted to various types of functional neurons by the addition of specific compounds.
What are IMR 32 cells?
A human neuroblastoma cell line (IMR-32), when differentiated, mimics large projections of the human cerebral cortex and under certain tissue culture conditions, forms intracellular fibrillary material, commonly observed in brains of patients affected with Alzheimer’s disease.
What are SH SY5Y neuroblastoma cells?
Is SK-N-SH a suitable target cell line for transfection?
SK-N-SH has been used as a target cell line in cell mediated cytotoxicity assays and is a suitable transfection host. The SK-N-SH line was developed by J.L. Biedler and differs from SK-N-MC (see ATCC HTB-10) in that it exhibits a longer doubling time and higher levels of dopamine – beta – hydroxylase.
What is SK-N-SH?
To schedule or learn more, read this. SK-N-SH is a neuroblastoma cell line that displays epithelial morphology and grows in adherent culture. Treatment with all-trans-retinoic acid causes these cells to differentiate and adopt a neuronal phenotype, characterized by extensive neurite outgrowth.
How do I get a certificate of analysis for SK-N-SH (htb-11)?
To download a certificate of analysis for SK-N-SH ( HTB-11 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of SK-N-SH ( HTB-11) is not currently available online. Complete this form to request this certificate of analysis.
What is the role of all-trans-retinoic acid in the pathophysiology of SK-N-SH cells?
Treatment with all-trans-retinoic acid causes these cells to differentiate and adopt a neuronal phenotype, characterized by extensive neurite outgrowth. This makes them particularly useful for delineating signaling pathways involved in neuronal differentiation. In addition, the SK-N-SH cells are known to form tumors in immunocompromised mice.